Preparation and quality evaluation of two types of colchicine ethosome patches / 中草药

Objective: To optimize the two types of colchicine ethosome patches using uniform design and to investigate their in vitro release, transdermal properties, stability, and stimulus. Methods: Two kinds of colchicine ethosomes made of soybean lecithin (SL) and hydrogenated phosphatidy (HP) were prepared using injection method. By the DPS 7.0 sotfware, the uniform design was used to optimize the patch components of colchicines, polyacrylic resin IV, succinic acid, triethyl citrate, and azone in patch pressure-sensitive adhesive, with its initial adhesion and hold tack as indexes. The colchicine ethosomes patches of SL and HP were prepared, respectively. The automated dissolution test analyzer and improved Franz diffusion cell were adopted to compare the in vitro release and transdermal properties of the two types of colchicine ethosomes patches, respectively. Moreover, the preparation stability under different light, humidity, and high temperature conditions was studied, and the skin irritation test for rabbits was carried out. Results: The optimized colchicine ethosome patches by the uniform design adhere with the viscous standards in Chinese Pharmacopeia 2010. The in vitro release of the three types of patches were fitted with the Higuchi equation and their release rates increased in turn, respectively, which were (3.1331 ± 0.3272), (2.8724 ± 0.3481), and (2.3802 ± 0.2134) μg/(cm2·h1/2). Their in vitro transdermal releases were fitted with the zero-class equation and increased in turn, respectively, which were (0.9390 ± 0.0731), (0.8775 ± 0.0908), and (0.6167 ± 0.0816) μg/(cm2·h). During the storage, the HP patches had better stability than the SL patches, especially with the stable color. In the skin irritation test for rabbits, there was no erythema and oedema reactions in 48 h. Conclusion: The HP patches have obviously higer in vitro release rate and transdermal rate, and for its better stability of preparation, the HP patches are superior to the SL ones.

Effect of Huzhang on expression of lung fibroblasts' MMP-2/TIMP-1mRNA in pulmonary fibrosis rats / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effect of Huzhang on the progress of pulmonary fibrosis in rats, evaluate the role of Huzhang in this process and explore its mechanism.</p><p><b>METHOD</b>Wistar male rats were randomized into 7 groups (normal control group, model group, positive control group, prophylactic group, 3rd day treatment group, 7th day treatment group and 14th day treatment group). Bleomycin was administered by intratracheal injection to produce pulmonary fibrosis groups except the normal control group. The positive control group began to be given DXM (4 mL x kg(-1) x d(-1)) on the day of the model-making. The normal control group and model group were given NS (4 mL x kg(-1) x d(-1)) on the day of the model-making. The prophylactic group was given reagent (4 mL x kg(-1) x d(-1)) 2 days ahead of the model-making, whereas the 3rd day treatment group, the 7th day treatment group and the 14th day treatment group given the same dose respectively on the third day, the seventh day and the fourth day behind of the model-making. Lung tissue was stained with hematoxylin-eosin (HE) and Masson's trichrome to determine the pathological grading. The lung fibroblast (LF) was cultured in vitro by way of pancreatic enzyme digestion, which was used to detect the contents of the expression of MMP-2 and TIMP-1mRNA with RT-PCR method.</p><p><b>RESULT</b>Compared with those in the model group, the alveolitis, pulmonary fibrosis and collagen accumulation were significantly alleviated in the positive control group, Huzhang prophylactic group and each treatment groups. In the positive control group, Huzhang prophylactic group, the 3rd day treatment group, the 7th day treatment group and the 14th day treatment group, the expression of MMP-2 mRNA was weaker significantly than that in the BLM model group (P < 0.05 or P < 0.01) except that on the 42nd day. The expression of TIMP-1mRNA was also weaker significantly than that in the BLM model group at all set times in all treatment groups (P < 0.05 or P < 0.01). The inhibition of TIMP-1 lasted until the 42nd day.</p><p><b>CONCLUSION</b>Huzhang inhibited the expression of MMP-2mRNA and TIMP-1mRNA of lung fibroblast in different periods to reduce the alveolitis and pulmonary fibrosis, which was probably one of the anti-fulmonary fiborsis mechanisms of Huzhang.</p>

Studies on constituents of Oldenlandia diffusa / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Oldenlandia diffusa.</p><p><b>METHOD</b>The column chromatography with polyamide Sephadex LH -20, silica gel as packing materials and HPLC, were used to separate and purify the chemical components. The structures were elucidated on the basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Nine compounds were isolated and identified as 2, 6-dihydroxy-1-methoxy-3-methylanthraquinone (1), 2-hydroxy-1-methoxy-3-methylanthraquinone (2), 2-hydroxy-3-methylanthraquinone (3), quercetin-3-O-[2-O-(6-O-E-sinapoyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (4), quercetin-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (5), kaempferol-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-galactopyranoside (6), quercetin-3-O-(2-O-beta-D-glucop-yranosyl)-beta-D-glucopyranoside (7), rutin (8) and quercertin (9).</p><p><b>CONCLUSION</b>Compounds 1 and 8 were obtained from this plant for the first time, and compound 1 was a new compound.</p>

The Clinical Study of the H1N1 Influenza Virus with Fanggan Decoction / 浙江中医药大学学报

[Objective]To discuss the clinical effect of Fanggan decoction on H1N1 Influenza virus.[Method]Randomly divided 95 cases into 2 groups,treatment group 48 and control one 47.Treatment group takes Fanggan decoction,the control one uses reducing fever tablet or anti-virus drugs if needed.[Result]The effective rate was 89.6% for treatment group and 85.1% for the control one.The treatment group was better than the control one on the reducing fever time,cough duration and cold symptom time.[Conclusion]Fanggan decoction has a good effect on H1N1 influenza virus.It also can be one of the therapies to relieve influenza patients in Zhejiang Province.